The P53 antibody is a tumor suppressor gene that is expressed in many tissue types and involved in regulations of cell replication, apoptosis, and growth. It will bind to the SV40T and MDM2 antigens, as well as the human papiulloma virus’s E6 protein. It can sense DNA damage and may help to repair any damages. Mutations can include a variety of malignant tumors, including the ovaries, bladder, breast, lung, colon, and melanoma.
The P53 antibody is designed for research needs only. It does have a clone called SPM514, and the isotype is IgG20/k. The immunogen is the recombinant human P53 protein. It has an undetermined epitope and a molecular weight of 53kDa.
This antibody is designed for Immunohistochemistry applications. To prepare your specimen, you will want to use paraffin-embedded or Formalin-fixed tissues. Slides should be deparaffinized using xylene, an alternative to xylene, and graded alcohols. If you choose the concentrate format of the product, you’ll want to dilute the antibody using a ratio of 1:400, though this is an estimate. Your protocol or method may require different dilutions. You can also find a pre-diluted format of this product, which is suitable for IHC applications.
To retrieve the antigen, you’ll need to boil the tissue sections in a 10mM Citrate buffer with a pH of 6.0. Do this for 10 minutes and allow the concoction to cool to room temperature for 20 minutes. There is also an incubation period of 30 minutes at room temperature before visualization.
The positive control is the colon carcinoma with cellular localization occurring in the nucleus.
The P53 antibody can be used to sense DNA damage and help with repairs, making it an excellent product to help with research. Visit Spring Bioscience now to learn more.