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How To Test For H. Pylori For Research Purposes

H. Pylori (Helicobacter pylori) can cause peptic ulcers or chronic gastritis in human beings. It is also associated with duodenal ulcers and could be involved in people developing adenocarcinoma or low-grade lymphoma of mucosa based on lymphoid tissues in the stomach. To test for this bacteria, it is essential that you perform the correct research. You’ll find antibodies available that can help you determine if the tissues are infected with H. pylori, which can help you determine which treatments are necessary.

This antibody is designed to be used for research and has a clone of SPM526. The immunogen is purified Helicobacter pylori, and it has an isotype of IgG1/k. It has no determined epitope and is designed to be used with Immunohistochemistry applications.

The Procedure

When using Immunohistochemistry, you will want to prepare your specimen using Formalin-fixed or paraffin-embedded tissues that are suitable. Deparaffinized slides are required for cleanliness and correct testing procedures, which means you can use xylene or an alternative, as well as graded alcohols.

If you do not choose to use the pre-diluted formulation, you will need to dilute the antibody using a ratio of 1:100, though pre-diluted forms are available for your convenience.

To retrieve the antigen, you should boil your tissue section in 10mM Citrate buffers with a pH of 6.0 for about 10 to 20 minutes. You should then let it cool to room temperature for 20 minutes. Incubation periods for H. Pylori are 30 minutes at room temperature, after retrieving the antigen. You can check for the bacteria using the instruction provided with your visualization system.

The positive control is a stomach with the Helicobacter pylori infection with the cellular localization being the cell wall of H. pylori.

The H. Pylori test is an excellent way to see if the tissue has that bacteria. Visit Spring Bioscience for purchase options.

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