Find Out More About The FOXP3 Antibody And Uses

by | Jan 5, 2017 | Science and Technology

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Technically, there are two FOXP3 antibody options, including the traditional and the monoclonal with a clone. Both versions are an amino acid protein and a member of the winged-helix/forkhead family of transcriptional regulators. They are highly conserved across all mammals. This antibody is essential for normal immune homeostasis. It is stable and constitutively expressed at high levels in CD35 + CD4 positive T-cells, and a low level in CD4 positive and CD25 negative cells. It is absent in CD4-negative and CD8-positive T-cells. It could be a master regulatory gene and a marker for other T-cells.

More Information About Each

Both FOXP3 antibodies are similar, though the FOXP3 monoclonal antibody has a clone of SP97. Both are used for research purposes only, and both have an immunogen of a synthetic peptide corresponding to the FOXP3 and C-terminus protein. Likewise, both antibodies use the rabbit iGG isotype and have an undetermined epitope. The molecular weight is 50 kDa, and both are human tested.

Applications And Procedures

Immunohistochemistry (IHC) is the application used for this protein. To prepare the specimen, it is best to use a paraffin-embedded tissue that is Formalin-fixed. Deparaffinization of the slides is required using a xylene or xylene alternative, along with graded alcohols.

When using a concentrated version of the antibody, it is best to dilute it with a ratio of 1:100, though your results may differ based on protocols and methods used.

Antigen retrieval is possible when you boil the tissue sections in EDTA with a pH of 8.0 for at least 10 minutes, allowing it to cool to room temperature for 20 minutes. It will also require an incubation period of 30 minutes while at room temperature.

The FOXP3 antibody comes in a polyclonal and monoclonal options, both of which are similar in use. Visit Spring Bioscience to learn more and choose the best one for your needs.